QTL
Biosystems, LLC. has developed a detection technique that combines the
benefits of solution/suspention phase formats with the simplicity of solid
phase/lateral flow assays.
In this approach, the assay is performed in the solution/suspention phase,
using magnetic microspheres as a solid capture support. A magnetic
separation is then performed to isolate the bound analyte from the remainder
of the solution. After a wash step is performed internally within the
cartridge, the fluorescence signal is directly read from surface-bound
analyte.
This technique delivers significant benefits:
-
Solution/suspension phase ensures optimum kinetic conditions
- Fomation of the pellet concentrates the analyte, improving detection
sensitivity
- Dynamic surface generation eliminates non-specific binding associated
with lateral flow assay
- Robustness and simplicity of chemistries can be easily adapted to new
bio-threat agents